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Mouse Succinate Dehydrogenase Complex Subunit C (SDHC) ELISA Kit

CAT: 0013-GTR18812772-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18812772-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Succinate Dehydrogenase Complex Subunit C (SDHC) .
Product Name Alternative
PGL3 ELISA Kit, CYB560, SDH3 ELISA Kit, CYBL ELISA Kit, QPs1 ELISA Kit, Integral membrane protein CII-3 ELISA Kit, Succinate-ubiquinone oxidoreductase cytochrome B large subunit ELISA Kit, Succinate dehydrogenase cytochrome b560 ELISA Kit
UniProt
Q9CZB0
Reactivity
Mouse
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.068 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SDHC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SDHC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SDHC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SDHC in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q9CZB0

Succinate dehydrogenase cytochrome b560 subunit, mitochondrial

C560_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9CZB0
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Sdhc
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q544Q9, Q99JP2, Q9DCU7
Protein keywords

Biological process

Electron transportTransportTricarboxylic acid cycle

Ligand

HemeIronMetal-binding

Cellular component

MembraneMitochondrionMitochondrion inner membrane

Technical term

Reference proteome

Domain

Transit peptideTransmembraneTransmembrane helix

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