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Mouse Apelin (APLN) ELISA Kit

CAT: 0013-GTR18812585-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18812585-01Size:48 Tests
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Description
Mouse Apelin (APLN) ELISA Kit
Product Name Alternative
XNPEP2 ELISA Kit, APEL ELISA Kit, APJ endogenous ligand ELISA Kit
UniProt
Q9R0R4
Reactivity
Mouse
Target
APLN
Field of Research
Cardiovascular Research
Assay Type
Competitive
Assay Performance Time
2h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
34.8 pg/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse APLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
125-8000 pg/mL

UniProtKB · Q9R0R4

Apelin

APEL_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9R0R4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Apln
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

AngiogenesisGastrulation

PTM

Cleavage on pair of basic residues

Molecular function

Developmental proteinHormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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