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Mouse Left/Right Determination Factor 2 (LEFTY2) ELISA Kit

CAT: 0013-GTR18813213-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18813213-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 2 (LEFTY2) .
Product Name Alternative
TGFB4 ELISA Kit, EBAF ELISA Kit, LEFTA ELISA Kit, LEFTYA ELISA Kit, Endometrial Bleeding Associated Factor ELISA Kit, Left-right determination factor A ELISA Kit, Transforming growth factor beta-4 ELISA Kit
UniProt
P57785
Reactivity
Mouse
Field of Research
Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
5.2 pg/mL
Notes
For research use only.
Applications Notes
Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LEFTY2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LEFTY2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LEFTY2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LEFTY2 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
15.63-1000 pg/mL

UniProtKB · P57785

Left-right determination factor 2

LFTY2_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P57785
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Lefty2
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q8BMF7
Protein keywords

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

CytokineDevelopmental proteinGrowth factor

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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