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Mouse Klotho Beta (KLb) ELISA Kit

CAT: 0013-GTR18812082-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18812082-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Klotho Beta (KLb) .
Product Name Alternative
Klotho beta-like protein ELISA Kit
UniProt
Q99N32
Reactivity
Mouse
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
6 pg/mL
Notes
For research use only.
Applications Notes
Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KLb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KLb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLb in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
15.63-1000 pg/mL

UniProtKB · Q99N32

Beta-klotho

KLOTB_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q99N32
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Klb
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
BKL, BetaKlotho
EC number
—
Processing
—
Secondary accessions
B2RQN8, Q920J2, Q99N31
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembrane

PTM

Glycoprotein

Technical term

Reference proteome

Domain

RepeatTransmembraneTransmembrane helix

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