Rat Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

CAT: 0013-GTR18811406-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0013-GTR18811406-01Size:48 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 2, Basic (FGF2) .

Product Name Alternative

B-FGF ELISA Kit, BFGF ELISA Kit, FGFB ELISA Kit, HBGH-2 ELISA Kit, Basic Fibroblast Growth Factor ELISA Kit, Heparin-binding growth factor 2 ELISA Kit

UniProt

P13109

Reactivity

Rat

Field of Research

Neuroscience; Cardiovascular Research; Stem Cell & Developmental Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

6.4 pg/mL

Notes

For research use only.

Applications Notes

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGF2 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

15.63-1000 pg/mL

UniProtKB · P13109

Fibroblast growth factor 2

FGF2_RAT · Rattus norvegicus

View on UniProt ↗

Primary accession: P13109
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Fgf2
Protein existence: 1: Evidence at protein level
Organism: Rattus norvegicus (Rat)
Taxonomy ID: 10116
Alternative names: FGF-2
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

Biological process

AngiogenesisDifferentiation

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Cellular component

NucleusSecreted

Technical term

Reference proteome

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Alternative Products

Catalog	Name
IMS01 -138-313	FGF2 \| Basic fibroblast growth factor
144522	FGF2 (Basic fibroblast growth factor)
049026	FGF2 (Fibroblast Growth Factor 2, Basic)
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TRV-048233-01	ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)