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Human Dipeptidase 2 (DPEP2) ELISA Kit

CAT: 0013-GTR18811179-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18811179-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidase 2 (DPEP2) .
Product Name Alternative
DPEP2, UNQ284/PRO323Dipeptidase 2
UniProt
Q9H4A9
Reactivity
Human
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Sensitivity
0.068 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DPEP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DPEP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DPEP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DPEP2 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q9H4A9

Dipeptidase 2

DPEP2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9H4A9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DPEP2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.13.19
Processing
Precursor
Secondary accessions
A0A024R6Y5, B2RCF8, B3KS59, I3L248, Q6UX92, Q8TC95
Protein keywords

Coding sequence diversity

Alternative splicing

Molecular function

DipeptidaseHydrolaseMetalloproteaseProtease

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Biological process

Lipid metabolism

Cellular component

Membrane

Ligand

Metal-bindingZinc

Technical term

Proteomics identificationReference proteome

Domain

Signal

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