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Human Caldesmon (CALD) ELISA Kit

CAT: 0013-GTR18810835-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810835-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caldesmon (CALD) .
Product Name Alternative
CALD1 ELISA Kit, CDM ELISA Kit, H-CAD ELISA Kit, L-CAD ELISA Kit, NAG22 ELISA Kit
UniProt
Q05682
Reactivity
Human
Field of Research
Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.3 ng/mL
Notes
For research use only.
Applications Notes
Standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CALD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CALD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CALD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CALD in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.79-50 ng/mL

UniProtKB · Q05682

Caldesmon

CALD1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q05682
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CALD1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
CDM
EC number
—
Processing
—
Secondary accessions
A8K0X1, Q13978, Q13979, Q14741, Q14742, Q9UD91
Protein keywords

Molecular function

Actin-bindingCalmodulin-bindingMuscle protein

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmCytoskeleton

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Technical term

Proteomics identificationReference proteome

Domain

Repeat

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