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Mouse Alpha-Endorphin (aEP) ELISA Kit

CAT: 0013-GTR18810399-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810399-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse Alpha-Endorphin (aEP) ELISA Kit
Product Name Alternative
AEP
Reactivity
Mouse
Target
AEP
Field of Research
Metabolism Research, Neuroscience, Signal Transduction
Assay Type
Competitive
Assay Performance Time
2h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
4.15 pg/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse aEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse aEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse aEP in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
15.63-1000 pg/mL

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