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Human Bridging Integrator 2 (BIN2) ELISA Kit

CAT: 0013-GTR18810197-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810197-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bridging Integrator 2 (BIN2) .
Product Name Alternative
BRAP-1 ELISA Kit, Breast cancer-associated protein 1 ELISA Kit
UniProt
Q9UBW5
Reactivity
Human
Field of Research
Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.107 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BIN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BIN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BIN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BIN2 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.32-20 ng/mL

UniProtKB · Q9UBW5

Bridging integrator 2

BIN2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9UBW5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BIN2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B7Z6F3, F5H0W4, Q86VV0, Q9NWK4, Q9UKN4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell junctionCell membraneCell projectionCytoplasmMembrane

Domain

Coiled coil

PTM

Phosphoprotein

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