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Human Complement Component 6 (C6) ELISA Kit

CAT: 0013-GTR18810062-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810062-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 6 (C6) .
Product Name Alternative
AW111623 , C6 , CO6_HUMAN , Complement component 6 , Complement component C6
UniProt
P13671
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
0.294 ng/mL
Notes
For research use only.
Applications Notes
Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C6 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.63-40 ng/mL

UniProtKB · P13671

Complement component C6

CO6_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P13671
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C6
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Complement pathwayCytolysisImmunityInnate immunity

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignalSushiTransmembraneTransmembrane beta strand

Cellular component

MembraneMembrane attack complexSecretedTarget cell membraneTarget membrane

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