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Human Interferon Alpha 2 (IFNa2) ELISA Kit

CAT: 0013-GTR18810005-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810005-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 2 (IFNa2) .
Product Name Alternative
IFNα2A ELISA Kit, IFNα2B ELISA Kit, IFNα2C ELISA Kit, LeIF A ELISA Kit, Alpha-2a Interferon ELISA Kit, Interferon Alpha 2b ELISA Kit, Interferon Alpha A ELISA Kit
UniProt
P01563
Reactivity
Human
Field of Research
Infectious Disease & Virology; Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
6.3 pg/mL
Notes
For research use only.
Applications Notes
Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNa2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNa2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNa2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNa2 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
15.63-1000 pg/mL

UniProtKB · P01563

Interferon alpha-2

IFNA2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01563
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IFNA2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IFN-alpha-2
EC number
—
Processing
Precursor
Secondary accessions
H2DF54, H2DF55, P01564, Q14606, Q6DJX8, Q96KI6
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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