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Mouse Lymphotoxin Beta (LTb) ELISA Kit

CAT: 0013-GTR18809989-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18809989-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphotoxin Beta (LTb) .
Product Name Alternative
LT-B ELISA Kit, p33 ELISA Kit, TNFC ELISA Kit, TNF-C ELISA Kit, TNFSF3 ELISA Kit, Tumor necrosis factor ligand superfamily member 3 ELISA Kit, Tumor Necrosis Factor C ELISA Kit
UniProt
P41155
Reactivity
Mouse
Field of Research
Cancer Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
6.5 pg/mL
Notes
For research use only.
Applications Notes
Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LTb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LTb in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
15.63-1000 pg/mL

UniProtKB · P41155

Lymphotoxin-beta

TNFC_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P41155
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ltb
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
LT-beta
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Molecular function

Cytokine

PTM

Glycoprotein

Cellular component

Membrane

Technical term

Reference proteome

Domain

Signal-anchorTransmembraneTransmembrane helix

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