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Human Apolipoprotein O (APOO) ELISA Kit

CAT: 0013-GTR18809905-02Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18809905-02Size:96 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein O (APOO) .
Product Name Alternative
Apo-O ELISA Kit, FAM121B ELISA Kit, My025 ELISA Kit, Family With Sequence Similarity 121B ELISA Kit
UniProt
Q9BUR5
Reactivity
Human
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.052 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOO in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q9BUR5

MICOS complex subunit MIC26

MIC26_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BUR5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APOO
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B2R4K9, Q9H3J9
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Endoplasmic reticulumGolgi apparatusHDLLDLMembraneMitochondrionMitochondrion inner membraneSecretedVLDL

PTM

GlycoproteinProteoglycan

Biological process

Lipid transportTransport

Technical term

Proteomics identificationReference proteome

Domain

SignalTransmembraneTransmembrane helix

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