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Human Envoplakin (EVPL) ELISA Kit

CAT: 0013-GTR18808925-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18808925-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Envoplakin (EVPL) .
Product Name Alternative
EVPK ELISA Kit, p210 ELISA Kit, IVL ELISA Kit, 210 kDa cornified envelope precursor protein ELISA Kit, 210 kDa paraneoplastic pemphigus antigen ELISA Kit
UniProt
Q92817
Reactivity
Human
Field of Research
Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.058 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EVPL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EVPL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EVPL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EVPL in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

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