Chicken Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

CAT: 0013-GTR18808328-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0013-GTR18808328-01Size:48 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 2, Basic (FGF2) .

Product Name Alternative

B-FGF ELISA Kit, BFGF ELISA Kit, FGFB ELISA Kit, HBGH-2 ELISA Kit, Basic Fibroblast Growth Factor ELISA Kit, Heparin-binding growth factor 2 ELISA Kit

UniProt

P48800

Reactivity

Gallus

Field of Research

Neuroscience; Cardiovascular Research; Stem Cell & Developmental Biology

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Serum, plasma and other biological fluids.

Sensitivity

6.4 pg/mL

Notes

For research use only.

Applications Notes

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken FGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken FGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken FGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken FGF2 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

15.63-1000 pg/mL

UniProtKB · P48800

Fibroblast growth factor 2

FGF2_CHICK · Gallus gallus

View on UniProt ↗

Primary accession: P48800
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: FGF2
Protein existence: 2: Evidence at transcript level
Organism: Gallus gallus (Chicken)
Taxonomy ID: 9031
Alternative names: FGF-2
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

Biological process

AngiogenesisDifferentiation

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

Cellular component

NucleusSecreted

Technical term

Reference proteome

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Alternative Products

Catalog	Name
144522	FGF2 (Basic fibroblast growth factor)
IMS01 -138-313	FGF2 \| Basic fibroblast growth factor
TRV-048233-01	ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
049026	FGF2 (Fibroblast Growth Factor 2, Basic)
MBS6404291-01	FGF2 (Basic fibroblast growth factor) (PE)
MBS6404285-01	FGF2 (Basic fibroblast growth factor) (HRP)
MBS6404282-01	FGF2 (Basic fibroblast growth factor) (APC)
MBS6404284-01	FGF2 (Basic fibroblast growth factor) (FITC)
MBS6404283-01	FGF2 (Basic fibroblast growth factor) (Biotin)
MBS6404281-01	FGF2 (Basic fibroblast growth factor) (AP)