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Rat Osteonectin (ON) ELISA Kit

CAT: 0013-GTR18808006-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18808006-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON) .
Product Name Alternative
SPARC ELISA Kit, BM-40 ELISA Kit, Secreted Protein, Acidic, Cysteine-Rich ELISA Kit, Basement-membrane protein 40 ELISA Kit, Secreted protein acidic and rich in cysteine ELISA Kit
UniProt
P16975
Reactivity
Rat
Field of Research
Cancer Biology, Gastroenterology & Hepatology, Infectious Disease & Virology, Metabolism Research, Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity
0.249 ng/mL
Notes
For research use only.
Applications Notes
Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ON. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ON. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ON, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ON in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.63-40 ng/mL

UniProtKB · P16975

SPARC

SPRC_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P16975
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Sparc
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
O08953, Q6GSZ4
Protein keywords

Cellular component

Basement membraneExtracellular matrixSecreted

Ligand

CalciumCopperMetal-binding

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Domain

Signal

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