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VPS35/MEM3 Antibody

CAT: 0013-GTR17696532Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17696532Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to VPS35
Product Name Alternative
Anti VPS35 antibody, anti MEM3 antibody, anti FLJ10752 antibody, anti FLJ13588 antibody, anti FLJ20388 antibody, anti DKFZp434E1211 antibody, anti DKFZp434P1672 antibody, anti vacuolar protein sorting 35 (yeast) antibody, anti maternal-embryonic 3 antibody, anti vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, anti vacuolar protein sorting 35 antibody
Reactivity
Human, Mouse, Rat
Target
VPS35 / MEM3
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
SPESEGPIYEGLIL
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
1-3 μg/ml
Dilution
ELISA: 1:128000, WB: 1.0-3.0 μg/ml, IHC-P: 5-8 μg/ml
Molecular Weight
91.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.WB: Approx 90kDa band observed in Human Brain and Testis lysates (predicted MW of 91kDa according to NP_060676.2) . Recommended for use at 1-3 μg/ml.Experiment Notes: Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please see Scott KL et al.; Nature. 2009 Jun 25;459 (7250) :1085-90.; PMID: 19553991.
Prediction Reactivity
Bovine
Tested Applications
ELISA, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Entrez
55737

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