Products for Research Use Only

Human Hephaestin (HEPH) ELISA Kit

CAT: 0013-GTR18810186-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18810186-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hephaestin (HEPH) .
Product Name Alternative
CPL ELISA Kit
UniProt
Q9BQS7
Reactivity
Human
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.58 ng/mL
Notes
For research use only.
Applications Notes
Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HEPH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HEPH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HEPH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HEPH in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
1.57-100 ng/mL

UniProtKB · Q9BQS7

Hephaestin

HEPH_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BQS7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HEPH
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Hp
EC number
1.16.3.1
Processing
Precursor
Secondary accessions
B1AJX8, D3DVT7, E9PHN8, O75180, Q6UW45, Q9C058
Protein keywords

Coding sequence diversity

Alternative splicing

Ligand

CalciumIronMetal-bindingSodium

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Biological process

Ion transportIron transportTransport

Molecular function

Oxidoreductase

Technical term

Proteomics identificationReference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix