Products for Research Use Only

CARD11 Antibody

CAT: 0013-GTR17696410Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17696410Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to CARD11
Product Name Alternative
Anti CARD11 antibody, member 11 antibody, anti BIMP3 antibody, anti CARMA1 antibody, anti MGC133069 antibody, anti card-maguk protein 1 antibody, anti bcl10-interacting maguk protein 3 antibody
Reactivity
Human
Target
CARD11
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QRKTIWVDEDQL
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-5 μg/ml
Molecular Weight
133
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration, 2-5ug/ml.WB: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2) . A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown) . These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
84433

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