MyoD1 Antibody

CAT: 0013-GTR18717138Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18717138Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

UniProt

P15172

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant mouse MyoD1 protein was used as the immunogen for this MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein.

Clonality

Monoclonal

Clone

5.8A

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Protein G affinity chromatography

Dilution

Flow Cytometry: 0.5-1ug/million cells in 0.1ml, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the MyoD1 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

UniProtKB · P15172

Myoblast determination protein 1

MYOD1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P15172
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: MYOD1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: O75321

Protein keywords

PTM

AcetylationMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorDevelopmental proteinDNA-binding

Biological process

DifferentiationMyogenesisTranscriptionTranscription regulation

Disease

Disease variant

Cellular component

Nucleus

Technical term

Proteomics identificationReference proteome

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