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CD99 Antibody

CAT: 0013-GTR18716848Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18716848Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32) .Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.
UniProt
P14209
Reactivity
Human
Immunogen
Human acute lymphocytic leukemia T-cells were used as the immunogen for the CD99 antibody.
Clonality
Monoclonal
Clone
12E7
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity
Dilution
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD99 antibody should be determined by the researcher.
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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