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CD166 Mouse Monoclonal Antibody

CAT: 0013-GTR18701854-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18701854-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD166 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Cultured human thymic epithelial cells .Purification: The antibody was purified by affinity chromatography.
Product Name Alternative
MEMD; CD166 antigen; Activated leukocyte cell adhesion molecule; CD166
UniProt
Q13740
Reactivity
Human
Immunogen
Cultured human thymic epithelial cells
Target
ALCAM
Clonality
Monoclonal
Clone
B5C8
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
The antibody was purified by affinity chromatography.
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .
Tested Applications
FC, IF
Host or Source
Mouse
Preservative
PBS, pH 7.3, and 0.02% sodium azide.
Isotype
Mouse IgG1 kappa
Entrez
214

UniProtKB · Q13740

CD166 antigen

CD166_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13740
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ALCAM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B2RNS3, B4DTU0, O60892, Q1HGM8, Q1HGM9, Q6PEY4, Q6ZS95
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Adaptive immunityCell adhesionImmunity

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCell projectionMembraneSecreted

PTM

Disulfide bondGlycoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

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