Tween 20

• Background:

  Non ionic detergents like Tween 20 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tween 20 or Polysorbate 20 PEG (20) sorbitan monolaurate, lowers surface tension of water.

• Description:

  Non ionic detergents like Tween 20 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction.

• Certification:

  RUO

• Other Statements:

  For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

• Label:

  ICT

• Type:

  IHC Reagents

• Applications:

  IHC, ELISA

• Assay Protocol:

  For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml) . Washing buffer use 1:500 (1 ml into 499 ml) . The optimum dilution should be determined by the individual lab. (NOT RECOMMENDED FOR DILUTION OF S100 ANTIBODIES)

• Buffer:

  This detergent is supplied as 50% in water with 0.15 % preservative.

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Conditions:

  Room Temperature

• Storage Temperature:

  Room temperature

• Target Description:

  Non ionic detergents like Tween 20 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tween 20 or Polysorbate 20 PEG(20) sorbitan monolaurate, lowers surface tension of water.