Eosin Y

• Background:

  Hematoxylin and Eosin (H&E) staining is used most commonly in histology. Hematoxylin is a basic dye and stains basophilic structure. Eosin Y is a fluorescent red dye is used to stain cytoplasm, collagen and muscle fiber. Slides stained with hematoxylin and eosin can only be mounted with organic mounting mediums. H&E stains slides are not compatible with aqueous mounting medium.

• Description:

  Hematoxylin and Eosin (H&E) staining is used most commonly in histology.

• Specifications:

  Ready to use counterstain

• Certification:

  RUO

• Other Statements:

  For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

• Label:

  ICT

• Type:

  IHC Reagents

• Applications:

  IHC

• Assay Protocol:

  H and E staining: Follow the procedure as outlined for H and E staining. We use the following protocol: 1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections. 2. Stain with hematoxylin for 4 minutes (2X), wash 4-5X with tap water, and rinse 2 X with de/di water. 3. Dip in 10 % acetic acid 2X, wash with de/di water, wash with Immuno Automation buffer (ImmunoBioScience AR-6561), PBS or any buffer with pH 7.4-8. 4. Rinse 2X with 70% ethanol, apply Eosin stain 1-5 minutes, and wash with 70% ethanol to remove excess Eosin.. 5. Rinse 2X with 95% ethanol, 2X with 100% ethanol, xylene or any dehydrating agent. 6. The slide can be mounted with organic mounting medium or our Organo /limonene (AR-6504) mounting medium.

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Conditions:

  Room Temperature

• Storage Temperature:

  Room temperature

• Target Description:

  Hematoxylin and Eosin (H&E) staining is used most commonly in histology. Hematoxylin is a basic dye and stains basophilic structure. Eosin Y is a fluorescent red dye is used to stain cytoplasm, collagen and muscle fiber. Slides stained with hematoxylin and eosin can only be mounted with organic mounting mediums. H&E stains slides are not compatible with aqueous mounting medium.