LDS-751

CAT: 0804-HY-D0996-01Size: 5 mgDry Ice: NoHazardous: No
CAT#:0804-HY-D0996-01Size:5 mg
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Description
Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 has a high affinity for DNA and fluorescence is enhanced after binding, but the maximum emission wavelength is 670nm. Lds-751 and Thiazole orange can be used for the differentiation of red blood cells, platelets, reticulocytes, and nucleated cells and can be stimulated at 488nm. Studies have shown that LDS-751 binds almost exclusively to mitochondria when incubated with nucleated living cells. After nucleated Acridine Orange (HY-101879) staining and LDS-751 treatment of cells, confocal microscopy revealed almost no co-location of the cells. Staining with Rhodamine 123 (HY-D0816), a dye known to bind polarized mitochondria, was almost identical to the pattern observed with LDS-751[1][2][3].
CAS Number
[181885-68-7]
UNSPSC
12171500
Target
DNA Stain
Type
Dye Reagents
Related Pathways
Cell Cycle/DNA Damage
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/LDS-751.html
Purity
99.30
Solubility
DMSO : 83.33 mg/mL (ultrasonic; warming; heat to 60°C)
Smiles
CC[N+]1=C2C=CC(N(C)C)=CC2=CC=C1/C=C/C=C/C3=CC=C(N(C)C)C=C3.O=Cl(=O)([O-])=O
Molecular Formula
C25H30ClN3O4
Molecular Weight
471.98
References & Citations
[1]Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123 (1) :103-12.|[2]Terstappen LW, et al. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry. 1988;9 (6) :548-556.|[3]McCarthy DA, et al. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods. 1993;163 (2) :155-160.|[4]Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123 (1) :103-12.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, sealed storage, away from moisture and light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

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