LDS-751

• Description :

  Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 has a high affinity for DNA and fluorescence is enhanced after binding, but the maximum emission wavelength is 670nm. Lds-751 and Thiazole orange can be used for the differentiation of red blood cells, platelets, reticulocytes, and nucleated cells and can be stimulated at 488nm. Studies have shown that LDS-751 binds almost exclusively to mitochondria when incubated with nucleated living cells. After nucleated Acridine Orange (HY-101879) staining and LDS-751 treatment of cells, confocal microscopy revealed almost no co-location of the cells. Staining with Rhodamine 123 (HY-D0816), a dye known to bind polarized mitochondria, was almost identical to the pattern observed with LDS-751[1][2][3].

• UNSPSC :

  12171500

• Target :

  DNA Stain

• Type :

  Dye Reagents

• Related Pathways :

  Cell Cycle/DNA Damage

• Field of Research :

  Others

• Assay Protocol :

  https://www.medchemexpress.com/LDS-751.html

• Purity :

  99.30

• Solubility :

  DMSO : 83.33 mg/mL (ultrasonic; warming; heat to 60°C)

• Smiles :

  CC[N+]1=C2C=CC(N(C)C)=CC2=CC=C1/C=C/C=C/C3=CC=C(N(C)C)C=C3.O=Cl(=O)([O-])=O

• Molecular Formula :

  C25H30ClN3O4

• Molecular Weight :

  471.98

• References & Citations :

  [1]Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123 (1) :103-12.|[2]Terstappen LW, et al. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry. 1988;9 (6) :548-556.|[3]McCarthy DA, et al. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods. 1993;163 (2) :155-160.|[4]Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123 (1) :103-12.

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  -20°C (Powder, sealed storage, away from moisture and light)

• Scientific Category :

  Dye Reagents

• Clinical Information :

  No Development Reported

• CAS Number :

  [181885-68-7]