Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (MALS verified)

• Background:

  Human cytomegalovirus (HCMV), a prototypical beta-herpes virus, is a major cause of morbidity and mortality in immunocompromised transplant recipients and congenitally infected fetuses.Different HCMV glycoprotein complexes are capable of meditating entry through interaction with cell-specific receptors on a panel of host cells. The pentamer (gH/gL/UL128/UL130/UL131A) mediates HCMV entry into epithelial, endothelial, and myeloid cells by its binding to neuropilin 2 (Nrp2) . The trimer (gH/gL/gO) can mediate infection of all cell types by binding to platelet-derived growth factor-alpha (PDGFRα) . Both pentamer and trimer need to interact with the glycoprotein B (gB), the fusogenic protein, to trigger the virus and host cell membrane fusion.

• Description:

  Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (GLO-V5243) is expressed from human 293 cells (HEK293) . It contains AA Lys 31 - Gln 466 & Val 31 - Arg 278 & Arg 24 - Leu 720 (Accession # P12824 & P16832 & P16750) .

• Specifications:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 162.2 kDa. The protein migrates as 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  P12824 & P16832 & P16750

• Expression Region:

  Lys 31 - Gln 466 & Val 31 - Arg 278 & Arg 24 - Leu 720

• Host:

  HEK293

• Target:

  Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV)

• Conjugation:

  Unconjugated

• Tag:

  N-10xHis

• Source:

  Human cytomegalovirus

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) at 1 μg/mL (100 μL/well) can bind Anti-HCMV gH [HCMV16], Human, IgG1 with a linear range of 0.05-2 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  162.2 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  50ug*1

• Species:

  Human cytomegalovirus