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Dog Haptoglobin (HP) ELISA Kit

CAT: 0013-GTR18863366-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863366-01Size:48 Tests
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Description
Dog Haptoglobin (HP) ELISA Kit
Product Name Alternative
HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin; Hpt; Haptoglobin
UniProt
P19006
Reactivity
Canine
Field of Research
Hematology, Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
0.62 μg/mL
Notes
For research use only.
Applications Notes
Standard: 100 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog HP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog HP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HP in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
1.56-100 μg/mL

UniProtKB · P19006

Haptoglobin

HPT_CANLF · Canis lupus familiaris

View on UniProt ↗
Primary accession
P19006
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HP
Protein existence
1: Evidence at protein level
Organism
Canis lupus familiaris (Dog)
Taxonomy ID
9615
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q9TRH6, Q9TRH7
Protein keywords

Biological process

Acute phaseImmunity

Molecular function

AntibioticAntimicrobialAntioxidantSerine protease homolog

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Ligand

Hemoglobin-binding

Cellular component

Secreted

Domain

Sushi

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