VEGFR-3/FLT-4

• Description:

  Receptor tyrosine kinase VEGFR-3, also known as Flt-4, together with VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1), are the receptors for vascular endothelial growth factors (VEGF) . The VEGFR family belongs to the class II subfamily of receptor tyrosine kinases (RTKs), containing a large extracellular region which is composed of seven Ig-like domains (D1–D7), a single transmembrane (TM) helix and cytoplasmic region with a tyrosine kinase activity. In VEGFR-3, the fifth Ig homology domain is proteolytically cleaved which results in polypeptides remaining linked by two disulfide bonds. VEGFR-3 is widely expressed on all endothelial cells in early embryogenesis, while, in adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. VEGF-C and VEGF-D activation of VEGFR-3 plays an important role in the formation of the lymphatic vessel system.

• Synonyms:

  Vascular endothelial growth factor receptor-3; Flt4; Chy; Flt-4; VEGFR-3

• NCBI Gene ID:

  14257

• UniProt:

  P35917

• Accession Number:

  NP_032055.1

• Accession Number mRNA:

  NM_008029

• Chromosomal Location:

  11 A5-B1; 11 29.69 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  No cross reactivity to mouse VEGFR-1 and VEGFR-2. Cross reactivity to other species has not been tested!

• Target Antigen:

  Mouse VEGFR-3 extracellular domain

• Clone:

  (#5F63)

• Applications:

  WB, IHC (P)

• Purification Method:

  Protein G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Bioactivity:

  Western blot: Use at 0.25 - 0.50 µg/ml; IHC (Paraffin sections) : Use at 0.4 - 2.0 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2