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VEGFR-2/KDR (D7), soluble

CAT: 0209-S01-002Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0209-S01-002Size:50 µg
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Description
Recombinant Human soluble Endothelial Growth Factor Receptor-2 (sKDR (D7) ) is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein consists of all 7 extracellular domains, which contain all the information necessary for high affinity ligand binding. The receptor monomers have a mass of approximately 116 kDa. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs) . They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4) . Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation. The binding of VEGF165 to VEGFR-2 is dependent on heparin.
Synonyms
Soluble vascular endothelial growth factor receptor-2 ; soluble CD309; soluble VEGF receptor-2; sKDR
NCBI Gene ID
3791
UniProt
P35968
Accession Number
NP_002244.1
Accession Number mRNA
NM_002253.2
Chromosomal Location
4q11-q12
Reactivity
Human
Cross Reactivity
Human
Sequence
ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGIPLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVIQAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLERVAPTITGNLENQTTSIGESIEVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFIIEGA
Assay Protocol
The lyophilized human sKDR is soluble in water and most aqueous buffers, it should be reconstituted in water or PBS to a concentration of not lower than 100µg/ml.
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit the VEGF165-induced proliferation in human umbilical vein endothelial (HUVE) cells.
Length
738
Form
Lyophilized
Buffer
25mM MES, 100mM NaCl; pH 5.5
Reconstitution
Water
Molecular Weight
116.0 kDa
Storage Conditions
The material is stable for greater than six months at -20° C to -70° C. After the first thawing it is recommended to aliquote the material, because repeated freeze-thaw cycles will decrease the activity. Store at 4°C not longer than 2 days. Avoid repeated freeze-thaw cycles.
Host or Source
Insect cells
N Terminal Sequence
ASVGLPSVSL

UniProtKB · P35968

Vascular endothelial growth factor receptor 2

VGFR2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P35968
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KDR
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
VEGFR-2
EC number
2.7.10.1
Processing
Precursor
Secondary accessions
A2RRS0, B5A925, C5IFA0, O60723, Q14178
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

AngiogenesisDifferentiationHost-virus interaction

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell junctionCell membraneCytoplasmCytoplasmic vesicleEndoplasmic reticulumEndosomeMembraneNucleusSecreted

Molecular function

Developmental proteinKinaseReceptorTransferaseTyrosine-protein kinase

PTM

Disulfide bondGlycoproteinPhosphoproteinUbl conjugation

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

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