VEGFR-2/Flk-1

• Description:

  The antibody recognizes mouse vascular endothelial growth factor receptor 2, alos known as CD309, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, and fetal liver kinase-1. Flk-1 is a member of the tyrosine protein kinase family, sub-family CSF-1/PDGF, that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. Flk-1 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels. Flk-1 is a receptor for VEGF-A or fully processed VEGF-C; ligand binding plays a key role in vascular development and vascular permeability.

• Synonyms:

  Soluble vascular endothelial growth factor receptor-2 ; sKdr; sLy73; sFlk-1; sVEGFR2; sVEGF receptor 2; skinase insert domain protein receptor

• NCBI Gene ID:

  16542

• UniProt:

  P35918

• Accession Number:

  ACJ66293.1

• Accession Number mRNA:

  EU884114

• Chromosomal Location:

  5 C3.3; 5 42.0 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Recombinant mouse esFlk-1 (RT Cat# S01-M04)

• Clone:

  Rabbit IgG

• Applications:

  WB

• Purification Method:

  Protein A purified

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  Western Blot: use at 1-5 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Rabbit