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VEGF165b

CAT: 0209-300-081Size: 5 µgDry Ice: NoHazardous: No
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CAT#:0209-300-081Size:5 µg
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Description
Vascular endothelial growth factor-A (VEGF-A) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. Humans express two sets of alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids. VEGF165 appears to be the most abundant and potent of the angiogenic isoform set, followed by VEGF121 and VEGF189. The anti-angiogenic or “b” set of isoforms is differentially spliced to contain six alternate amino acids at the C-terminus, and are the more highly expressed isoforms in normal adult tissue. VEGF165b, like VEGF121 but unlike most angiogenic isoforms, does not bind heparins and is therefore diffusible. VEGFs bind the type I transmembrane receptor tyrosine kinases VEGFR1 (Flt1) and VEGFR2 (Flk1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to Flt1, KDR appears to be the primary mediator of VEGF angiogenic activity. The affinity of VEGF165b for KDR is similar to that of VEGF165, but VEGF165b only partially activates KDR such that the kinase regulatory site Y1054 is not phosphorylated. VEGF165b also does not bind Neuropilin-1, suggesting that the functional difference between VEGF165 and VEGF165b may be due to either the lack of Neuropilin-1 co-signaling or unique downstream signaling activated by VEGF165b. Since VEGF165b may compete with angiogenic VEGFs for KDR sites, its ectopic expression in tumors has been shown to inhibit their growth.
Synonyms
Vascular endothelial growth factor A; VEGFA; VPF; VEGF; MVCD1
NCBI Gene ID
7422
UniProt
P15692-8
Accession Number
NP_001165100.1
Accession Number mRNA
NM_001171629.1
Chromosomal Location
6p12
Reactivity
Human
Cross Reactivity
Human
Sequence
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRSLTRKD
Assay Protocol
The lyophilized VEGF165 should be reconstituted in 50 mM acetic acid to a concentration not lower than 50 µg/ml. For long term storage we recommend to add at least 0.1% human or bovine serum albumin.
Endotoxin
< 0.1 ng per µg of human VEGF165b
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA: Recombinant human sKDR (Cat# S01-004) and sKDR (D7) /Fc (Cat# SFC-008) were coated to the plate (1μg/ml) and rhVEGF165 and rhVEGF165b were added. Bound VEGF165 and VEGF165b were detected by an anti-human VEGF-A antibody.
Length
165
Form
Lyophilized
Buffer
50mM acetic acid
Reconstitution
50mM acetic acid
Molecular Weight
38.2 kDa
Storage Conditions
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF165 should be stored in working aliquots at -20°C.
Host or Source
E. coli
N Terminal Sequence
APMAEGG

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