TPO

• Description:

  TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein.

• Synonyms:

  DDX4; VASA

• NCBI Gene ID:

  54314

• UniProt:

  P49745

• Accession Number:

  NP_112395.1

• Accession Number mRNA:

  NM_031133.2

• Chromosomal Location:

  6q16

• Reactivity:

  Rat

• Cross Reactivity:

  Rat, Human

• Sequence:

  SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to stimulate the proliferation of human MO7e cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

• Length:

  174

• Form:

  Lyophilized

• Molecular Weight:

  18.7 kDa

• Host or Source:

  E. coli