TNFSF1A (TNF-alpha)

• Description:

  Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) . Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

• Synonyms:

  Tnf; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha

• NCBI Gene ID:

  21926

• UniProt:

  P06804

• Accession Number:

  NP_038721.1

• Accession Number mRNA:

  NM_013693.2

• Chromosomal Location:

  17 B1; 17 19.06 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Mouse recombinant protein TNFSF1A

• Clone:

  (#7F34)

• Applications:

  WB

• Purification Method:

  Protein A/G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2