T-cell Immunoglobulin Mucin-3 (TIM-3)

• Description:

  T cell immunoglobulin mucin-3 (TIM-3) is a member of the T-cell Immunoglobulin- and Mucin-domain-containing family of type I membrane glycoproteins that regulate autoimmune and allergic disease. TIM-3 is selectively expressed on Th1 cells and interacts with galectin-9. It negatively regulates Th1 responses and affects macrophage activation. The 280 aa mature human TIM-3 contains a V-type Ig-like domain that shows multiple polymorphisms, followed by a mucin-like domain in the 171 aa extracellular region, which shares 60% amino acid identity with mouse TIM-3 ECD. One splice variant of TIM-3 is truncated within the mucin domain and presumably is secreted.

• Synonyms:

  Havcr2; Tim3; TIM-3; Timd3

• NCBI Gene ID:

  171285

• UniProt:

  Q8VIM0

• Accession Number:

  NP_599011.2

• Accession Number mRNA:

  NM_134250.2

• Chromosomal Location:

  11 B1.1; 11

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Mouse recombinant protein of TIM-3

• Clone:

  (#6F46)

• Applications:

  WB

• Purification Method:

  Protein A/G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2