SPARC/Osteonectin Recombinant Protein

SPARC/Osteonectin Recombinant Protein

• Description: SPARC/Osteonectin is a secreted, evolutionarily conserved collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains; an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high affinity, and a cysteine-rich follistatin-like domain. Recombinant human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDS-PAGE analysis due to the effect of glycosylation.
• Synonyms: SPARC; ON
• CAS Number: 9000-83-3
• NCBI Gene ID: 6678
• UniProt: P09486
• Accession Number: NP_003109.1
• Accession Number mRNA: NM_003118.3
• Gene Location: 5q31.3-q32
• Host: CHO
• Origin Species: Human
• Species Reactivity: Rat
• Sequence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI
• Detection Range: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 cells, with an IC50 of 0.5-0.7 μg/ml.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 286
• Form: Lyophilized
• Molecular Weight: 43.7 kDa
• Shipping Conditions: Room Temperature