ROR2, soluble

• Description:

  ROR2 is a signaling receptor for Wnt ligands that is known to play important roles in limb development, but having no essential roles known in adult tissues. Recent evidence has implicated ROR2 in mediating both canonical and non-canonical signaling pathways. ROR2 was initially found to be highly expressed in osteosarcoma and renal cell carcinomas, and has recently been found in an increasingly long list of cancers currently including melanoma, colon cancer, melanoma, squamous cell carcinoma of the head and neck, and breast cancer. In the majority of these cancer types, ROR2 expression is associated with more aggressive disease states, consistent with a role mediating Wnt signaling regardless of the canonical or non-canonical signal. Because of the pattern of tissue distribution, the association with high-risk diseases, and the cell surface localization of this receptor, ROR2 has been identified as a potential high value target for therapeutic development. However, the recent discovery that ROR2 may function through non-kinase activities challenges this strategy and opens up opportunities to target this important molecule through alternative means.

• Synonyms:

  Neurotrophic tyrosine kinase, receptor-related 2

• NCBI Gene ID:

  4920

• UniProt:

  Q01974

• Accession Number:

  NP_004551.2

• Accession Number mRNA:

  NM_004560.3

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  EVEVLDPNDP LGPLDGQDGP IPTLKGYFLN FLEPVNNITI VQGQTAILHC KVAGNPPPNV RWLKNDAPVV QEPRRIIIRK TEYGSRLRIQ DLDTTDTGYY QCVATNGMKT ITATGVLFVR LGPTHSPNHN FQDDYHEDGF CQPYRGIACA RFIGNRTIYV DSLQMQGEIE NRITAAFTMI GTSTHLSDQC SQFAIPSFCH FVFPLCDARS RTPKPRELCR DECEVLESDL CRQEYTIARS NPLILMRLQL PKCEALPMPE SPDAANCMRI GIPAERLGRY HQCYNGSGMD YRGTASTTKS GHQCQPWALQ HPHSHHLSST DFPELGGGHA YCRNPGGQME GPWCFTQNKN VRMELCDVPS CSPRDSSKMG HHHHHH

• Assay Protocol:

  Centrifuge vial prior to opening. The lyophilized sROR2 is soluble in water and most aqueous buffers and should be reconstituted in water to a concentration not lower than 100 µg/ml.

• Purity:

  > 98% by SDS-PAGE & Coomassie stain

• Bioactivity:

  Measured by its binding ability in a functional ELISA. Immobilized human sROR2 at 2 μg/mL (100 μL/well) can bind rhWnt5a.

• Length:

  376

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Molecular Weight:

  50.0 kDa

• Storage Conditions:

  Lyophilized samples are stable for greater than six months at –20°C to –70°C. Reconstituted sROR2 should be stored in working aliquots at -70°C.

• Host or Source:

  Insect cells

• N Terminal Sequence:

  EVEVL