RANKL, soluble Recombinant Protein

RANKL, soluble Recombinant Protein

• Description: RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells. These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK + T-cells, and regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant mouse sRANK Ligand is a 19.8 kDa polypeptide comprising the TNF-homologous region of RANKL (178 amino acid residues).
• Synonyms: soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), OPGL, ODF (Osteoclast Differentiation Factor), CD254
• CAS Number: 9000-83-3
• NCBI Gene ID: 21943
• UniProt: O35235
• Accession Number: NP_035743.2
• Accession Number mRNA: NM_011613
• Gene Location: 14 D3; 14 45.0 cM
• Host: CHO
• Origin Species: Mouse
• Species Reactivity: Mouse
• Sequence: FSGAPAMMEG SWLDVAQRGK PEAQPFAHLT INAASIPSGS HKVTLSSWYH DRGWAKISNM TLSNGKLRVN QDGFYYLYAN ICFRHHETSG SVPTDYLQLM VYVVKTSIKI PSSHNLMKGG STKNWSGNSE FHFYSINVGG FFKLRAGEEI SIQVSNPSLL DPDQDATYFG AFKVQDID
• Detection Range: Determined by its ability to activate LacZ reporter under control of NF-kappa-B-responsive element in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 3.0-20.0 ng/ml.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Length: 178
• Form: Lyophilized
• Molecular Weight: 19.8 kDa
• Shipping Conditions: Room Temperature