Prolactin

• Description :

  Recombinant ovine prolactin, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (see Leibovich et al., Protein Expr Purif. 2001 22:489-96) .

• Synonyms :

  Mammotropin, Luterotropic hormone, Lutetropin

• NCBI Gene ID :

  443317

• UniProt :

  P01240

• Accession Number :

  NP_001009306.1

• Accession Number mRNA :

  NM_001009306.1

• Reactivity :

  Ovine

• Cross Reactivity :

  Ovine

• Sequence :

  ATPVCPNGPGNCQVSLRDLFDRAVMVSHYIHNLSSEMFNEFDKRYAQGKGFITMALNSCHTSSLPTPEDKEQAQQTHHEVLMSLILGLLRSWNDPLYHLVTEVRGMKGVPDAILSRAIEIEEENKRLLEGMEMIFGQVIPGAKETEPYPVWSGLPSLQTKDEDARHSAFYNLLHCLRRDSSKIDTYLKLLNCRIIYNNNC

• Assay Protocol :

  It is recommended to reconstitute the lyophilized oPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 99% by SDS-PAGE & HPLC analyses

• Bioactivity :

  Recombinant oPRL is fully biologically active as evidenced by inducing proliferation of Nb2 cells.

• Length :

  199

• Form :

  Lyophilized

• Reconstitution :

  Water

• Molecular Weight :

  23.0 kDa

• Host or Source :

  E. coli

• N Terminal Sequence :

  ATPVCP