Prolactin

• Description:

  Recombinant ovine prolactin, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (see Leibovich et al., Protein Expr Purif. 2001 22:489-96) .

• Synonyms:

  Mammotropin, Luterotropic hormone, Lutetropin

• NCBI Gene ID:

  443317

• UniProt:

  P01240

• Accession Number:

  NP_001009306.1

• Accession Number mRNA:

  NM_001009306.1

• Reactivity:

  Ovine

• Cross Reactivity:

  Ovine

• Sequence:

  ATPVCPNGPGNCQVSLRDLFDRAVMVSHYIHNLSSEMFNEFDKRYAQGKGFITMALNSCHTSSLPTPEDKEQAQQTHHEVLMSLILGLLRSWNDPLYHLVTEVRGMKGVPDAILSRAIEIEEENKRLLEGMEMIFGQVIPGAKETEPYPVWSGLPSLQTKDEDARHSAFYNLLHCLRRDSSKIDTYLKLLNCRIIYNNNC

• Assay Protocol:

  It is recommended to reconstitute the lyophilized oPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 99% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Recombinant oPRL is fully biologically active as evidenced by inducing proliferation of Nb2 cells.

• Length:

  199

• Form:

  Lyophilized

• Reconstitution:

  Water

• Molecular Weight:

  23.0 kDa

• Host or Source:

  E. coli

• N Terminal Sequence:

  ATPVCP