Prolactin Recombinant Protein

• Description:

  Recombinant chickenn prolactin, (chPRL) one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques as described by Oclon et al (2017) GCE 240, 27-34.

• Synonyms:

  Mammotropin, Luterotropic hormone, Lutetropin

• NCBI Gene ID:

  396453

• UniProt:

  P14676

• Accession Number:

  NP_990797.2

• Accession Number mRNA:

  NM_205466.3

• Host:

  E. coli

• Origin Species:

  Chicken

• Species Reactivity:

  Chicken

• Sequence:

  ALPICPIGSVNCQVSLGELFDRAVKLSHYIHYLSSEIFNEFDERYAQGRGFITKAVNGCHTSSLTTPEDKEQAQQIHHEDLLNLVVGVLRSWNDPLIHLASEVQRIKEAPDTILWKAVEIEEQNKRLLEGMEKIVGRVHSGDAGNEIYSHWDGLPSLQLADEDSRLFAFYNLLHCLRRDSHKIDNYLKVLKCRLIHDSNC

• Detection Range:

  Recombinant hPRL is biologically active as evidenced by inducing proliferation of Nb2 cells and baf/3 cells stably transfected with hPRL receptors and by in vivo in chicken.

• Endotoxin:

  < 0.1 ng/µg of protein (<1EU/µg)

• Purity:

  > 95% by SDS-PAGE & HPLC analyses

• Length:

  199

• Form:

  Lyophilized

• Reconstitution:

  It is recommended to reconstitute the lyophilized chPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

• Molecular Weight:

  23.0 kDa

• Shipping Conditions:

  Room Temperature

• N Terminal Sequence:

  ALPICP

• CAS Number:

  9000-83-3