Podocalyxin

• Description:

  Podocalyxin is the major sialoprotein in the glycocalyx of glomerular podocytes. It was shown that endothelial cell membranes contain Podocalyxin. By immunoelectron microscopy Podocalyxin was localized on the luminal membrane domain of endothelial cells, in a patchy distribution. The presence of Podocalyxin was confirmed in SDS extracts of lung tissue by immunoblotting. It is concluded that (a) Podocalyxin is a widespread component of endothelial plasma membranes, (b) it is restricted to the luminal membrane domain, and (c) it is distributed unevenly on the endothelial cell surface.

• Synonyms:

  PODXL; PC; PCLP; Gp200; PCLP-1; Podocalyxin-like protein, GCTM-2 antigen, Gp200

• NCBI Gene ID:

  5420

• UniProt:

  O00592

• Accession Number:

  NP_001018121

• Accession Number mRNA:

  NM_001018111

• Chromosomal Location:

  7q32-q33

• Reactivity:

  Anti-Human

• Cross Reactivity:

  Human

• Target Antigen:

  Membrane protein fraction of isolated human glomeruli

• Clone:

  (#GB6D9C11)

• Applications:

  IHC (P) ; IF

• Purification Method:

  Protein G affinity chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

• Bioactivity:

  IF/IHC: Use at 2-5 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  Water

• Storage Conditions:

  The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

• Host or Source:

  Mouse

• Isotype:

  IgG1