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Podocalyxin

CAT: 0209-101-M142Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0209-101-M142Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Podocalyxin is the major sialoprotein in the glycocalyx of glomerular podocytes. It was shown that endothelial cell membranes contain Podocalyxin. By immunoelectron microscopy Podocalyxin was localized on the luminal membrane domain of endothelial cells, in a patchy distribution. The presence of Podocalyxin was confirmed in SDS extracts of lung tissue by immunoblotting. It is concluded that (a) Podocalyxin is a widespread component of endothelial plasma membranes, (b) it is restricted to the luminal membrane domain, and (c) it is distributed unevenly on the endothelial cell surface.
Synonyms
PODXL; PC; PCLP; Gp200; PCLP-1; Podocalyxin-like protein, GCTM-2 antigen, Gp200
NCBI Gene ID
5420
UniProt
O00592
Accession Number
NP_001018121
Accession Number mRNA
NM_001018111
Chromosomal Location
7q32-q33
Reactivity
Anti-Human
Cross Reactivity
Human
Target Antigen
Membrane protein fraction of isolated human glomeruli
Clone
(#GB6D9C11)
Applications
IHC (P) ; IF
Purification Method
Protein G affinity chromatography
Assay Protocol
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Bioactivity
IF/IHC: Use at 2-5 µg/ml.
Form
Lyophilized
Buffer
PBS
Reconstitution
Water
Storage Conditions
The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
Host or Source
Mouse
Isotype
IgG1