PlGF

CAT: 0209-M30-019SSize: 2 µgDry Ice: NoHazardous: No
CAT#:0209-M30-019SSize:2 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra and interchain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high affinity to Flt1, but not to Flk1/KDR.
Synonyms
Pgf; PlGF; Plgf; AI854365; placental growth factor
NCBI Gene ID
18654
UniProt
P49764
Accession Number
NP_032853
Accession Number mRNA
NM_008827
Chromosomal Location
12 D; 12 39.0 cM
Reactivity
Mouse
Cross Reactivity
Mouse
Sequence
ALSAGNNSTEVEVVPFNEVWGRSYCRPMEKLVYILDEYPDEVSHIFSPSCVLLSRCSGCCGDEGLHCVPIKTANITMQILKIPPNRDPHFYVEMTFSQDVLCECRPILETTKAERRKTKGKRKRSRNSQTEEPHP
Assay Protocol
Centrifuge vial prior to opening. The lyophilised PlGF is supplied in lyophilized form with carrier-protein (BSA) and can be reconstituted with 50mM acetic acid or PBS/water. This solution can be diluted into other buffered solutions or stored frozen for future use.
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. Recombinant mouse PlGF can bind to immobilized rh-sFlt-1 (100 ng/well) with a linear range at 0.5 - 10 ng/mL.
Length
135/132
Form
Lyophilized
Buffer
25 mM Tris, 75 mM NaCl pH 8.5
Additives
BSA (50-fold)
Reconstitution
50 mM acetic acid
Molecular Weight
~40 kDa
Storage Conditions
The lyophilized mouse PIGF, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C.
Host or Source
Insect cells
N Terminal Sequence
ALSAGNNSTE and AGNNSTE

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