Mouse Anti-Human PDGFR-alpha Monoclonal Antibody
CAT:
209-101-M824
Size:
100 µg
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Mouse Anti-Human PDGFR-alpha Monoclonal Antibody
- Description: PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfidelinked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF Rα binds all three PDGF isoforms with high affinity, PDGF Rβ binds PDG-FBB and AB, but not PDGFAA. Both PDGF Rα and PDGF Rβ are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for MCSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the α and β receptors is independently regulated in various cell types. Only PDGF Rα is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGFRα has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF Rα binds PDGF with high affinity and is a potent PDGF antagonist.
- Synonyms: PDGFRA; CD140A; PDGFR2; PDGFR-2; RHEPDGFRA
- CAS Number: 9007-83-4
- NCBI Gene ID: 5156
- UniProt: P16234
- Accession Number: NP_006197.1
- Accession Number mRNA: NM_006206
- Gene Location: 4q12
- Host: Mouse
- Origin Species: Human
- Species Reactivity: Human
- Target Antigen: N-terminal Recombinant Human PDGFR alpha fragment
- Isotype: IgG1
- Clone: (#8A12)
- Validated Applications: IP, FC
- Purification Method: Protein G chromatography
- Form: Lyophilized
- Buffer: PBS
- Reconstitution: Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.
- Shipping Conditions: Room Temperature
- Storage Conditions: Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity.