Osteopontin Recombinant Protein

Osteopontin Recombinant Protein

• Description: Osteopontin is a secreted glycoprotein that functions as a ligand to alphavbeta3 integrin and possibly other receptors. It binds tightly to hydroxyapatite and can act as a structural component of the extracellular mineralized matrix. Osteopontin is initially secreted as a 298 amino acid protein, which is subject to multiple post-translational modifications including glycosylation, phosphorylation, and specific proteolytic cleavages into various smaller molecular weight fragments. Osteopontin is expressed in a wide range of cells and tissues including osteoblasts, various tumor cell lines, and extraosseous cells in the inner ear, brain, kidney, deciduum, placenta and odontoblasts. In addition to its involvement in mineralized matrix formation, Osteopontin can also function as a cytokine that stimulates the release of IFNγ and IL-12, while inhibiting the production of IL-10. Recombinant human Osteopontin is a 298 amino acid protein, which, due to glycosylation, migrates at an apparent molecular weight of 60-65 kDa by SDS-PAGE analysis under reducing conditions. Recombinant human Osteopontin has a calculated, theoretical molecular weight of 33.7 kDa.
• Synonyms: SPP1; OPN; BNSP; BSPI; ETA-1
• CAS Number: 9000-83-3
• NCBI Gene ID: 6696
• UniProt: P10451
• Accession Number: NP_001035147
• Accession Number mRNA: NM_001040058
• Gene Location: 4q22.1
• Host: HEK 293
• Origin Species: Human
• Species Reactivity: Human
• Sequence: IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN
• Detection Range: Determined by its ability to enhance cell adhesion of murine B16-F1 cells.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 97% by SDS-PAGE & HPLC analyses
• Length: 298
• Form: Lyophilized
• Buffer: 10mM Sodium Phosphate, pH 7.0
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Molecular Weight: 60-65 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.