NP-1 Recombinant Protein

NP-1 Recombinant Protein

• Description: Defensins (α and β) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins which include NP-1, NP-2 and NP-3, are distinguished from the β-defensins by the pairing of their three disulfide bonds. In addition to antimicrobial activity, NP-1 exhibits chemotactic activity on dendritic cells. NP-1 is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. NP-1 contains a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human NP-1 is a 3.4 kDa protein containing 30 amino acid residues.
• Synonyms: Neutrophil Peptide-1, alpha Defensin-1, HNP-1, Cryptdin
• CAS Number: 9000-83-3
• NCBI Gene ID: 8829
• UniProt: Q15818
• Accession Number: NP_002513.2
• Accession Number mRNA: NM_002522.3
• Gene Location: 10p12
• Host: E. coli
• Origin Species: Human
• Species Reactivity: Human
• Sequence: ACYCRIPACI AGERRYGYCI YQGRLWAFCC
• Detection Range: Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0-10.0 ng/ml.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 30
• Form: Lyophilized
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Molecular Weight: 3.4 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.