Neuropoietin

• Description:

  Neuropoietin is a newly identified member of the IL-6 cytokine family. Members of this family, including IL-6, IL-11, oncostatin M, leukemia inhibitory factor (LIF), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine, and CNTF, display a four-helix bundle structure, and signal through gp130-containing receptor complexes. Neuropoietin, which is predominantly expressed in neuroepithelia during embryonic life, acts through a receptor complex comprising CNTF receptor-a component, gp 130, and LIF receptor. Like CNTF, it promotes the survival of embryonic motor neurons and could increase the proliferation of neural precursor cells in the presence of EGF and FGF-2. Interestingly, the human Neuropoietin gene has evolved toward a pseudogene, suggesting that the alternative signaling via CNTF is an effective compensatory pathway. Recombinant murine Neuropoietin is a 19.8 kDa protein containing 183 amino acid residues.

• Synonyms:

  Ctf2; NP; Gm494

• NCBI Gene ID:

  244218

• UniProt:

  P83714

• Accession Number:

  NP_942155

• Accession Number mRNA:

  NM_198858

• Chromosomal Location:

  7 F3; 7

• Reactivity:

  Mouse

• Cross Reactivity:

  Mouse

• Sequence:

  MAPISPSEPI GQAYSLALYM QKNTSALLQT YLQHQGSPFS DPGFSAPELQ LSTLPSAAVS FKTWHAMEDA ERLSRAQGAF LALTQHLQLV GDDQSYLNPG SPILLAQLGA ARLRAQGLLG NMAAIMTALG LPIPPEEDTL GFVPFGASAF ERKCRGYIVT REYGHWTDRA VRDLALLKAK YSA

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analysis

• Bioactivity:

  The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.5-0.8 μg/ml.

• Length:

  183

• Form:

  Lyophilized

• Molecular Weight:

  19.8 kDa

• Host or Source:

  E. coli