Nesfatin

• Description:

  Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the precursor protein, Nucleobindin2 (NUCB2) . Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic isletsβ-cells, gastric endocrine cells and adipocytes. Nesfatin-1 suppresses food intake and can regulate energy metabolism in a Leptin independent manner. Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues.

• Synonyms:

  Nesfatin, Nucleobindin 2 protein

• NCBI Gene ID:

  4925

• UniProt:

  P80303

• Accession Number:

  NP_005004

• Accession Number mRNA:

  NM_005013.2

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Sequence:

  VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analysis

• Bioactivity:

  Determined by an in vivo assay using healthy wild type male mice (C57BL/6J) . Mice were treated via intraperitoneal injection once at a dose of 4μg Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.

• Length:

  82

• Form:

  Lyophilized

• Buffer:

  10mM Sodium Phosphate, pH 6.5

• Reconstitution:

  Water

• Molecular Weight:

  9.7 kDa

• Host or Source:

  E. coli