Marapsin

• Description:

  Marapsin, Pancreasin, and channel-activating protease 2 (CAP2), encoded by the Prss27 gene, are different names given for the same serine protease that is expressed strongly in the pancreas. The mouse protein is synthesized with a signal peptide (amino acid residues 1-22), a pro peptide (residues 23-37) and amature chain (residues 38-290) corresponding to the serine protease domain. The fulllengthprotein was expressed and the secreted protein purified. The N-terminalsequencing results indicate that the purified protein is a disulfide bond-linked dimer formed between the pro peptide and the mature chain. The active enzyme has lowactivity against peptide substrates tested, but high activity against thioester substrates. The peptidase activity is inhibited by 20 mM benzamidine.

• Synonyms:

  Prss27; Mpn; CAPH2; marapsin

• NCBI Gene ID:

  213171

• UniProt:

  Q8BJR6

• Accession Number:

  NP_780649.1

• Accession Number mRNA:

  NM_175440.4

• Chromosomal Location:

  17 A3.3; 17 10.6 cM

• Reactivity:

  Anti-Mouse

• Cross Reactivity:

  Mouse

• Target Antigen:

  Mouse recombinant protein of Marapsin

• Clone:

  (#9D66)

• Applications:

  WB

• Purification Method:

  Protein A/G chromatography

• Assay Protocol:

  Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

• Form:

  Lyophilized

• Buffer:

  PBS

• Reconstitution:

  PBS

• Storage Conditions:

  Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.

• Host or Source:

  Rat

• Isotype:

  IgG2