MANF

• Description:

  MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Recombinant human MANF is an 18.1 kDa protein consisting of 158 amino acids including 8 cysteine residues.

• Synonyms:

  MANF; ARP; ARMET

• NCBI Gene ID:

  7873

• UniProt:

  P55145

• Accession Number:

  NP_006001.3

• Accession Number mRNA:

  NM_006010.4

• Chromosomal Location:

  3p21.1

• Reactivity:

  Human

• Cross Reactivity:

  Human

• Target Antigen:

  Protein

• Sequence:

  LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 15-25 μg/ml.

• Length:

  158

• Form:

  Lyophilized

• Molecular Weight:

  18.1 kDa

• Host or Source:

  E. coli