LIGHT

• Description:

  LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTβR, or bind to a decoy receptor, DcR3. It is expressed in splenocytes, activated PBL, CD+8 tumor infiltrating lymphocytes, granulocytes, and monocytes. LIGHT has the ability to active NFkB, to co-stimulate the activation of lymphocytes and to induce apoptosis in certain human tumor cells. Recombinant murine LIGHT is a 20.1 kDa protein containing 183 amino acid residues.

• Synonyms:

  Tnfsf14; LTg; HVEML; LIGHT; Ly113; HVEM-L; LIGHT; tumor necrosis factor (ligand) superfamily, member 14

• NCBI Gene ID:

  50930

• UniProt:

  Q9QYH9

• Accession Number:

  NP_062291

• Accession Number mRNA:

  NM_019418

• Chromosomal Location:

  17; 17 D-E1

• Reactivity:

  Mouse

• Cross Reactivity:

  Human, Mouse

• Sequence:

  MRLHQRLGDI VAHLPDGGKG SWEKLIQDQR SHQANPAAHL TGANASLIGI GGPLLWETRL GLAFLRGLTY HDGALVTMEP GYYYVYSKVQ LSGVGCPQGL ANGLPITHGL YKRTSRYPKE LELLVSRRSP CGRANSSRVW WDSSFLGGVV HLEAGEEVVV RVPGNRLVRP RDGTRSYFGA FMV

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its cytotoxic effecton HT-29 cells (human colon adenocarcinoma cells) in the presence of human IFN-gamma.

• Length:

  183

• Form:

  Lyophilized

• Molecular Weight:

  20.1 kDa

• Host or Source:

  E. coli