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LIF

CAT: 0209-M30-008Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0209-M30-008Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant mouse LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.86 kDa.
Synonyms
Lif; leukemia inhibitory factor
NCBI Gene ID
16878
UniProt
P09056
Accession Number
NP_032527
Accession Number mRNA
NM_008501
Chromosomal Location
11 A1-A2; 11 00.25 cM
Reactivity
Mouse
Cross Reactivity
Mouse
Sequence
SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF
Assay Protocol
The lyophilized LIF should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
Endotoxin
< 0.1 ng per µg (IEU/µg) of rm LIF
Purity
> 98% by SDS-PAGE
Bioactivity
The ED50 as determined by the M1 cell differentiation assay is in the range of 0.1 - 0.5 ng/ml.
Length
180
Form
Lyophilized
Buffer
0.5X PBS
Reconstitution
Water
Molecular Weight
19.86 kDa
Storage Conditions
The lyophilized LIF, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted LIF should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .
Host or Source
E. coli
N Terminal Sequence
SPLPIT

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